IN VITRO FERTILIZATION ( IVF TREATMENT )


This is the most widely practiced assisted conception procedure in the world and in simple terms means fertilizing the eggs outside the body and transferring the resulting embryos in the uterus for implantation.

 

INDICATION OF IVF-ET

 

Female factors

IVF INDIA - Infertility Treatment INDIA

1) Bilateral tubal obstruction
2) Premature ovarian failure

3) Severe endometriosis
4) Poor responder to ovulation induction

Male Factors

1) Severe oligozoospermia
2) Oligoasthernozoospermia
3) Psychological dysfunction etc.

Unexplained Causes:

STEPS OF IVR-ET

1) Ovulation induction
2) Egg retrieval
3) Sperm preparation and insemination
4) EMBRYO transfer


PROTOCOL USED

long / short protocol. In the long protocol, treatment is started on days 21 of previous cycle, GnRH agonist is started on day 21 to down regulate the ovary.

 

OVULATION INDUCTION

The goal is to stimulate the ovaries to produce several follicles. In the normal cycle only one egg is mature and ovulates. The various drugs used are:

1) GnRH agonist
2) Human Menopausal gonadotropins (HMG)
3) Purified follicle stimulation hormone (FSH)
4) Recombinant FSH

The response is monitored by the frequent ultrasound and frequent blood levels of estrogens. The aim is to achieve between 5-10 follicles of 15-18 mm size. When this is achieved HCG in the dose of 5000 to 10000 IU is administered by intramuscular injection. The eggs pick up is done about 32-35 hours after HCG injection.

EGG RETRIEVAL

It is done under anesthesia using intravenous Pentothal with the help of a vaginal ultrasound. There is no cut or operation. Under the guidance, the needle is inserted into the follicle. The follicles are aspirated and the aspirate is examined in the lab for the eggs. This is a day care procedure and the patient is sent after 2-3 hours. One may experience some vaginal spotting or pain in abdomen, which subsides on its own. Complications from the procedures in <1%.

 

SPERM INSEMINATION

The eggs are placed in the incubator for 4-6 hours and are then inseminated with motile sperms that have been prepared by washing techniques.

 

EMBRYO TRANSFER

Embryo takes between 24-48 hours to develop. Between 24 and 72 hours after fertilization the embryo is ready to be replaces into the uterus. Patients are advised to rest for about 4 hours and after going home to rest for 3-4 days. Progesterone support is given and blood test is done 14 days after the ET procedure. This can is the form of injection or a vaginal or oral tablets. HCG is also used to supplement progesterone in cases where there is no ovarian hyuperstimulation.

IVF SUCCESS - IVF SUCCESS RATE

The reported PREGNANCY rate in an IVF-ET procedure is 40% and take home baby rate is 30-35% per cycle. There is no increase in the rate of foetal malformation as compared to normal conception.

 

IVF TREATMENT AT GAUDIUM Infertility TREATMENT INDIA

All the above steps are executed under personal guidance of  DR MANIIKA KHANNA for each & every case. STRICT STANDARD OPERATING PROTOCOLS (SOP’S) are used for the entire staff at GAUDIUM. Discipline regarding critical timings for certain points in the IVF cycle like timing of HCG administration & timing of OVAM PICKUP are strictly adhere to our staff. We also expect the patient to comply with these timing very strictly.